Nucleotide sequence of wheat germ cytoplasmic initiator methionine transfer ribonucleic acid
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Initiator Methionine Transfer Ribonucleic Acid from Wheat Embryo
Initiator methionine tRNA (tRNAIMet) species from wheat embryo has been purified by fractionation on a benzoylated DEAE-cellulose column followed by chromatography on a DEAE-Sephadex column. Transfer RNAIMet can be aminoacylated by the homologous enzyme as well as by Escherichia coli aminoacyl-tRNA synthetase. Unlike other eukaryotic cytoplasmic initiator tRNAs, the wheat embryo initiator tRNA ...
متن کاملInitiator methionine transfer ribonucleic acid from wheat embryo. Purification, properties, and partial nucleotide sequences.
Initiator methionine tRNA (tRNAIMet) species from wheat embryo has been purified by fractionation on a benzoylated DEAE-cellulose column followed by chromatography on a DEAE-Sephadex column. Transfer RNAIMet can be aminoacylated by the homologous enzyme as well as by Escherichia coli aminoacyl-tRNA synthetase. Unlike other eukaryotic cytoplasmic initiator tRNAs, the wheat embryo initiator tRNA ...
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The nucleotide sequence analysis of tRNA has required (a) identification of fragments isolated from complete digestion with pancreatic RNase and RNase T1 and, in addition, (b) the analysis of large oligonucleotide fragments produced by partial digestion with RNase T1 (l-4) and, in some cases, partial digestion with pancreatic RNase (5). In continuation of the work reported in the preceding pape...
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An amino acid-incorporating system has been obtained from ungerminated wheat germ, which can be stimulated by the addition of uridylic-rich polynucleotides. Polyadenylic and polycytidylic acids fail to stimulate the incorporation of lysine and proline, respectively. The amino acid codons determined by the use of the uridylic-rich polynucleotides coincide almost perfectly with those reported for...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1982
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/10.10.3241